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hsyn1 promoter  (Addgene inc)


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    Addgene inc hsyn1 promoter
    CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( <t>hSYN1</t> ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.
    Hsyn1 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hsyn1 promoter - by Bioz Stars, 2026-02
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    1) Product Images from "The Citron homology domain of MAP4Ks improves outcomes of traumatic brain injury"

    Article Title: The Citron homology domain of MAP4Ks improves outcomes of traumatic brain injury

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-24-00113

    CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( hSYN1 ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.
    Figure Legend Snippet: CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( hSYN1 ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.

    Techniques Used: Gene Expression, Fluorescence, Immunostaining, Virus, Immunohistochemistry



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    CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( <t>hSYN1</t> ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.
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    CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( <t>hSYN1</t> ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.
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    CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( <t>hSYN1</t> ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.
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    CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( <t>hSYN1</t> ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.
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    Image Search Results


    CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( hSYN1 ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.

    Journal: Neural Regeneration Research

    Article Title: The Citron homology domain of MAP4Ks improves outcomes of traumatic brain injury

    doi: 10.4103/NRR.NRR-D-24-00113

    Figure Lengend Snippet: CNH in neurons exerts neuroprotection. (A) Experimental design to examine CNH function in astrocytes. Gene expression is controlled by the astrocyte-specific human GFAP ( hGFAP ) promoter. (B) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (C) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 5 mice per group; P = 0.1767). (D) Representative images showing AT8 surrounding the cortical lesion. Scale bar: 50 µm. (E) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 5 mice per group; P = 0.7153). (F) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (G) Quantification of GFAP + scar volumes (mean ± SEM; n = 5 mice per group; P = 0.7448). (H) Representative images of brain sections for lesion quantification (yellow outlined). Scale bar: 1 mm. (I) Quantification of cortical volumes post injury (mean ± SEM; n = 5 mice per group; P = 0.9039). (J) Experimental design to examine CNH function in neurons. Gene expression is controlled by the neuron-specific human SYN1 ( hSYN1 ) promoter. (K) Representative images showing GFAP + cells surrounding the cortical lesion. Scale bar: 50 µm. (L) Quantification of fluorescence intensity of GFAP (mean ± SEM; n = 6 mice per group; P = 0.0007). (M) Representative images showing immunostaining of AT8 surrounding the cortical lesion. Scale bar: 50 µm. (N) Quantification of AT8 fluorescence intensity (mean ± SEM; n = 6 mice per group; P = 0.0152). (O) Representative images of brain sections showing GFAP + glial scar (yellow outlined). Scale bar: 1 mm. (P) Quantification of GFAP + scar volumes (mean ± SEM; n = 6 mice per group; P = 0.0042). (Q) Representative images of brain sections for lesion quantifications (yellow outlined). Scale bar: 1 mm. (R) Quantification of cortical volumes post injury (mean ± SEM; n = 6 mice per group; P = 0.0227). AAV: Adeno-associated virus; Br: bregma; CCI: controlled cortical impact; CNH: Citron homology domain; dpi: days post-injury; GFAP: glial acidic fibrillary acidic protein; IHC: immunohistochemistry.

    Article Snippet: The following plasmids were obtained from Addgene (Watertown, MA, USA): pAd-deltaF6 (#112867), pUCmini-iCAP-PHP.eB (#103005), pAAV2/5 (#104964), pAAV2/9 (#112865), and pAAV-CAG-GFP (#37825). pAAV-hGFAP-GFP was previously described (Wang et al., 2021). pAAV-hSYN1-GFP was made by replacing the hGFAP promoter with the hSYN1 promoter (from Addgene #50457). pAAV-CAG-GFP-CNH , pAAV-CAG-BioID2-CNH , pAAV-hGFAP-CNH , and pAAV-hSYN1-CNH were then constructed through PCR-based subcloning into pAAV-CAG-GFP , pAAV-hGFAP-GFP and pAAV-hSYN1-GFP , respectively.

    Techniques: Gene Expression, Fluorescence, Immunostaining, Virus, Immunohistochemistry